Everything in Biology is Mechanical

Ephemeral states in protein folding under force captured with a magnetic tweezers design. Rafael Tapia-Rojo, Edward C. Eckels, and Julio M. Fernandez. PNAS (2019) (pdf)Download

The Mechanical Power of Titin Folding. Edward C. Eckels, Shubhasis Haldar, Rafael Tapia-Rojo, Jaime Andres Rivas-Pardo, and Julio M. Fernandez. CELL REPORTS. (2019) (pdf)Download

The Work of Titin Protein Folding as a Major Driver in Muscle Contraction. Edward C. Eckels, Rafael Tapia-Rojo, Jaime Andres Rivas-Pardo, and Julio M. Fernandez. ANNU. REV. PHYSIOL.. 80, 327-51 (2018) (pdf)Download

Molecular strategy for blocking isopeptide bond formation in nascent pilin proteins. Jaime Andres Rivas-Pardo, Carmen L. Badilla, Rafael Tapia-Rojo, Alvaro Alonso-Caballero, and Julio M. Fernandez. PNAS, 115, 37 (2018) (pdf)Download

Real-time Detection of Thiol Chemistry in Single Proteins. Edward C. Eckels, Daniel J. Echelman, Jaime Andres Rivas-Pardo, and Julio M. Fernandez.Oxidative Folding of Proteins- Basic Principles, Cellular Regulation, and Engineering (RSC Publishing) July, (2018) (pdf)Download

Disulfide isomerization reactions in titin immunoglobulin domains enable a mode of protein elasticity. David Giganti, Kevin Yan, Carmen L. Badilla, Julio M. Fernandez, and Jorge Alegre-Cebollada.NATURE COMMUNICATIONS, 9-185 (2018) (pdf)Download

Trigger factor chaperone acts as a mechanical foldase. Shubhasis Haldar, Rafael Tapia-Rojo, Edward C. Eckels, Jessica Valle-Orero, and Julio M. Fernandez. NATURE COMMUNICATIONS 8- 668, (2018) (pdf)Download

Proteins Breaking Bad: A Free Energy Perspective. Jessica Valle-Orero, Rafael Tapia-Rojo, Edward C. Eckels, Jaime Andres Rivas-Pardo, Ionel Popa, and Julio M. Fernandez. J. PHYS. CHEM. LETT. 8, 3642−3647 (2017) (pdf)Download

Mechanical deformation accelerates protein ageing. Jessica Valle-Orero, Jaime Andres Rivas-Pardo, Rafael Tapia-Rojo, Ionel Popa, Daniel J. Echelman, Shubhasis Haldar, and Julio M. Fernandez. ANGEW. CHEM. INT. ED., 56, 1 – 7 (2017) (pdf)Download

Mechanical forces regulate the reactivity of a thioester bond in a bacterial adhesin. Daniel J. Echelman, Alex Q. Lee, and Julio M. Fernández. J. BIOL. CHEM. 292, 8988 –8997 (2017) (pdf)Download

A HaloTag Anchored Ruler for Week-Long Studies of Protein Dynamics. Ionel Popa, Jaime Andres Rivas-Pardo, Edward C. Eckels, Daniel J. Echelman, Carmen L. Badilla, Jessica Valle-Orero, and Julio M. Fernandez. JACS, 138, 10546−10553 (2016) (pdf)Download

CnaA domains in bacterial pili are efficient dissipaters of large mechanical shocks. Daniel J. Echelman, Jorge Alegre-Cebollada, Carmen L. Badilla, Chungyu Chang, Hung Ton-That, and Julio M. Fernandez. PNAS, 113, 2490–2495 (2016) (pdf)Download

Work Done by Titin Protein Folding Assists Muscle Contraction. Jaime Andres Rivas-Pardo, Edward C Eckels, Ionel Popa, Pallav Kosuri, Wolfgang A. Linke, and Julio M. Fernandez. CELL REPORTS, 14, 1339–1347, (2016) (pdf)Download

Single-molecule Force Spectroscopy Predicts a Misfolded, Domain-swapped Conformation in human D-Crystallin Protein. Sergi Garcia-Manyes, David Giganti, Carmen L. Badilla, Ainhoa Lezamiz, Judit Perales-Calvo, Amy E. M. Beedle, and Julio M. Fernandez. J. BIOL. CHEM., 291, 8 (2016) (pdf)Download

Identifying Sequential Substrate Binding at the Single-Molecule Level by Enzyme Mechanical Stabilization. Jaime Andres Rivas Pardo, Jorge Alegre-Cebollada, Cesar A. Ramirez-Sarmiento, Julio M. Fernandez, and Victoria Guixe. ACS NANO, 9, 3996–4005, (2015) (pdf)Download

Monitoring oxidative folding of a single protein catalyzed by the disulfide oxidoreductase DsbA. Thomas B. Kahn, Julio M. Fernandez, and Raul Perez-Jimenez. J. BIOL. CHEM. 29, 23 (2015) (pdf)Download

The Elastic Free Energy of a Tandem Modular Protein Under Force. Jessica Valle-Orero, Edward C. Eckels, Guillaume Stirnemann, Ionel Popa, Ronen Berkovich, and Julio M. Fernandez. BBRC, 460, 434-438, (2015) (pdf)Download

Dynamics of Equilibrium Folding and Unfolding Transitions of Titin Immunoglobulin Domain under Constant Forces. Hu Chen, Guohua Yan, Ricksen S. Winardhi, Mingxi Yao, Ionel Popa, Julio M. Fernandez, and Jie Yan. JACS, 137, 3540−3546, (2015) (pdf)Download

A Novel Strategy for Utilizing Voice Coil Servoactuators in Tensile Tests of Low Volume Protein Hydrogels. Farees Saqlain, Ionel Popa, Julio M. Fernandez, and Jorge Alegre-Cebollada. MACROMOL. MATER. ENG. 300, 369–376 (2015) (pdf)Download

Probing the Effect of Force on HIV-1 Receptor CD4. Raul Perez-Jimenez, Alvaro Alonso-Caballero, Ronen Berkovich, David Franco, Ming-Wei Chen, Patricia Richard, Carmen L. Badilla, and Julio M. Fernandez. ACS NANO. VOL. 8, 10313–10320, (2014) (pdf)Download

Altered Thiol Chemistry in Human Amyotrophic Lateral Sclerosis-linked Mutants of Superoxide Dismutase. Carles Solsona, Thomas B. Kahn, Carmen L. Badilla, Cristina Alvarez-Zaldiernas, Juan Blasi, Julio M. Fernandez, and Jorge Alegre-Cebollada. J. BIOL. CHEM., 289, 39 (2014) (pdf)Download

S-Glutathionylation of Cryptic Cysteines Enhances Titin Elasticity by Blocking Protein Folding. Jorge Alegre-Cebollada, Pallav Kosuri, David Giganti, Edward Eckels Jaime Andres Rivas-Pardo, Nazha Hamdani, Chad M. Warren, R. John Solaro, Wolfgang A. Linke, and Julio M. Fernandez. Cell, 156, 1235–1246, (2014) (pdf)Download

Conservation of Protein Structure over Four Billion Years. Alvaro Ingles-Prieto, Beatriz Ibarra-Molero, Asuncion Delgado-Delgado, Raul Perez-Jimenez, Julio M. Fernandez, Eric A. Gaucher, Jose M. Sanchez-Ruiz, and Jose A. Gavira. STRUCTURE, 21, 1690–1697, (2013) (pdf)Download

Nanomechanics of HaloTag Tethers. Ionel Popa, Ronen Berkovich, Jorge Alegre-Cebollada, Carmen L. Badilla, Jaime Andres Rivas-Pardo, Yukinori Taniguchi, Masaru Kawakami, and Julio M. Fernandez. JACS, 135, 12762-12771, (2013) (pdf)Download

Conformational Plasticity of the Essential Membrane-associated Mannosyltransferase PimA from Mycobacteria. David O. Giganti, Jorge Alegre-Cebollada, Saioa Urresti, David Albesa-Jove, Ane Rodrigo-Unzueta, Natalia Comino, Michael Kachala, Sonia Lopez-Fernandez, Dmitri I. Svergun, Julio M. Fernandez, and Marcelo E. Guerin. J. BIOL. CHEM., 288, 29797-808 (2013) (pdf)Download

Force dependency of biochemical reactions measured by single-molecule force-clamp spectroscopy. Ionel Popa, Pallav Kosuri, Jorge Alegre-Cebollada, Sergi Garcia-Manyes, and Julio M Fernandez. NATURE PROTOCOLS, 8, 1261–1276, (2013) (pdf)Download

Elasticity, structure, and relaxation of extended proteins under force. Guillaume Stirnemann, David Giganti, Julio M. Fernandez, and B. J. Berne. PNAS, 110, 3847–3852 (2013) (pdf)Download

Protein Folding Drives Disulfide Formation. Pallav Kosuri, Jorge Alegre-Cebollada, Jason Feng, Anna Kaplan, Alvaro Ingles-Prieto, Carmen L. Badilla, Brent R. Stockwell, Jose M. Sanchez-Ruiz, Arne Holmgren, and Julio M. Fernandez. CELL, 151, 794–806 (2012) (pdf)Download

Rate limit of protein elastic response is tether dependent. Ronen Berkovich, Rodolfo I. Hermans, Ionel Popa, Guillaume Stirnemann, Sergi Garcia-Manyes, Bruce J. Berne, and Julio M. Fernandez. PNAS, 109, 14416–14421 , (2012) (pdf)Download

Spontaneous Dimerization of Titin Protein Z1Z2 Domains Induces Strong Nanomechanical Anchoring. Sergi Garcia-Manyes, Carmen L. Badilla, Jorge Alegre-Cebollada, Yalda Javadi, Julio M. Fernandez. J. BIOL. CHEM., 287, 24, (2012) (pdf)Download

Direct observation of disulfide isomerization in a single protein. Jorge Alegre-Cebollada, Pallav Kosuri, Jaime Andres Rivas-Pardo, and Julio M. Fernandez. NATURE CHEMISTRY, 3, 882–887 (2011) (pdf)Download

Direct Quantification of the Attempt Frequency Determining the Mechanical Unfolding of Ubiquitin Protein. Ionel Popa, Julio M. Fernandez, and Sergi Garcia-Manyes. J. BIOL. CHEM., 286, 36, (2011) (pdf)Download

Single-molecule paleoenzymology probes the chemistry of resurrected enzymes. Raul Perez-Jimenez, Alvaro Inglés-Prieto, Zi-Ming Zhao, Inmaculada Sanchez-Romero, Jorge Alegre-Cebollada, Pallav Kosuri, Sergi Garcia-Manyes, T Joseph Kappock, Masaru Tanokura, Arne Holmgrem, Jose M Sanchez-Ruiz, Eric A Gaucher, and Julio M Fernandez. NATURE STRUCT. & MOL. BIOL., 18, 592–596 (2011) (pdf)Download

Protease Power Strokes Force Proteins to Unfold. Jorge Alegre-Cebollada, Pallav Kosuri, and Julio M. Fernandez. CELL 145, (2011) (pdf)Download

Contrasting the Individual Reactive Pathways in Protein Unfolding and Disulfide Bond Reduction Observed within a Single Protein. Sergi Garcia-Manyes, Tzu-Ling Kuo, and Julio M. Fernandez. JACS, 133, 3104–3113, (2011) (pdf)Download

Kinetic Measurements on Single-Molecule Disulfide Bond Cleavage. Jian Liang, and Julio M. Fernandez. JACS, 133, 3528–3534, (2011) (pdf)Download

Hopping around an entropic barrier created by force. Ronen Berkovich, Sergi Garcia-Manyes, Joseph Klafter, Michael Urbakh, Julio M. Fernández. BBRC, 403, 133–137 (2010) (pdf)Download

Water’s role in the force-induced unfolding of ubiquitin. Jingyuan Lia, Julio M. Fernandez, and B. J. Berne. PNAS, 107, 19284–19289, (2010) (pdf)Download

Role of conservative mutations in protein multi-property adaptation. David Rodriguez-Larrea, Raul Perez-Jimenez, Inmaculada Sanchez-Romero, Asuncion, Delgado-Delgado, Julio M. Fernandez, and Jose M. Sanchez-Ruiz. BIOCHEM.. J. 429, 243–249, (2010) (pdf)Download

Single-molecule force spectroscopy approach to enzyme catalysis. Jorge Alegre-Cebollada, Raul Perez-Jimenez, Pallav Kosuri, and Julio M. Fernandez. J. BIOL. CHEM., 285, 25 (2010) (pdf)Download

Probing static disorder in Arrhenius kinetics by single-molecule force spectroscopy. Jingyuan LiItay Barel, Hui Lu, Bruce J. Berne, Michael Urbakh, Joseph Klafter, and Julio M. Fernandez. PNAS, 107, 11336–11340 (2010) (pdf)Download

Collapse Dynamics of Single Proteins Extended by Force. Ronen Berkovich, Sergi Garcia-Manyes, Michael Urbakh, Joseph Klafter, and Julio M. Fernandez. BIOPHYS. J. 98, 2692–2701 (2010) (pdf)Download

Isopeptide bonds block the mechanical extension of pili in pathogenic Streptococcus pyogenes. Jorge Alegre-Cebollada, Carmen L. Badilla, and Julio M. Fernandez. J. BIOL. CHEM. 285, 15 (2010) (pdf)Download

Extended Kalman filter estimates the contour length of a protein in single molecule atomic force microscopy experiments. Vicente I. Fernandez, Pallav Kosuri, Vicente Parot, and Julio M. Fernandez. REV. SCI. INST. 80, 113104 (2009) (pdf)Download

Modulation of Titin-Based Stiffness by Disulfide Bonding in the Cardiac Titin N2-B Unique Sequence. Anika Grützner, Sergi Garcia-Manyes, Sebastian Kötter, Carmen L. Badilla, Julio M. Fernandez, and Wolfgang A. Linke. BIOPHYS. J. 97, 825–834 (2009) (pdf)Download

Diversity of chemical mechanisms in thioredoxin catalysis revealed by single-molecule force spectroscopy. Raul Perez-Jimenez, Jingyuan Li, Pallav Kosuri, Inmaculada Sanchez-Romero, Arun P Wiita, David Rodriguez-Larrea, Ana Chueca, Arne Holmgreno, Antonio Miranda-Vizuete, Katja Becker, Seung-Hyun Cho, Jon Beckwith, Eric Gelhaye, Jean P Jacquot, Eric Gaucher, Jose M Sanchez-Ruiz, Bruce J Berne, and Julio M Fernandez. NAT. STRUCT. MOL. BIOL. 16, 890–896 (2009) (pdf)Download

Mechanochemistry: One Bond at a Time. Jian Liang, and Julio M. Fernandez. ACS NANO, 3, 1628-45, (2009) (pdf)Download

Direct observation of an ensemble of stable collapsed states in the mechanical folding of ubiquitin. Sergi Garcia-Manyes, Lorna Dougan, Carmen L. Badilla, Jasna Brujic, and Julio M. Fernandez. PNAS, 106, 10534-10539 (2009) (pdf)Download

Osmolyte-induced separation of the mechanical folding phases of ubiquitin. Sergi Garcia-Manyes, Lorna Dougan, and Julio M. Fernandez. PNAS, 06, 10540-10545, (2009) (pdf)Download

Force-activated reactivity switch in a bimolecular chemical reaction. Sergi Garcia-Manyes, Jian Liang, Robert Szoszkiewicz, Tzu-Ling Kuo, and Julio M. Fernandez. NAT. CHEM. 1, 236–242 (2009) (pdf)Download

Mechanical Characterization of Protein L in the Low-Force Regime by Electromagnetic Tweezers/Evanescent Nanometry. Ruchuan Liu, Sergi Garcia-Manyes, Atom Sarkar, Carmen L. Badilla, and Julio M. Fernandez. BIOPHYS. J. 96, 3810–3821, (2009) (pdf)Download

Stretching Single Talin Rod Molecules Activates Vinculin Binding. Armando del Rio, Raul Perez-Jimenez, Ruchuan Liu, Pere Roca-Cusachs, Julio M. Fernandez, and Michael P. Sheetz. SCIENCE, 323, 638-641, (2009) (pdf)Download

Force-Clamp Spectroscopy Detects Residue Co-evolution in Enzyme Catalysis. Raul Perez-Jimenez, Arun P. Wiita, David Rodriguez-Larrea, Pallav Kosuri, Jose A. Gavira, Jose M. Sanchez-Ruiz, and Julio M. Fernandez. J. BIOL. CHEM. 283, 27121–27129 (2008) (pdf)Download

Single-Molecule Force Spectroscopy Measurements of Bond Elongation during a Bimolecular Reaction. Sri Rama Koti Ainavarapu, Arun P . Wiita, Lorna Dougan, Einar Uggerud, and Julio M . Fernandez. JACS, , 130, 6479–6487 (2008) (pdf)Download

Dwell Time Analysis of a Single-Molecule Mechanochemical Reaction. Robert Szoszkiewicz, Sri Rama Koti Ainavarapu, Arun P. Wiita, Raul Perez-Jimenez, Jose M. Sanchez-Ruiz, and Julio M. Fernandez. LANGMUIR. 24, 1356-1364. (2008) (pdf)Download

A Single-Molecule Assay to Directly Identify Solvent-Accessible Disulfide Bonds and Probe Their Effect on Protein Folding. Sri Rama Koti Ainavarapu, Arun P. Wiita, Hector H. Huang, and Julio M. Fernandez. JACS. 130, 436-437, (2008) (pdf)Download

Probing the chemistry of thioredoxin catalysis with force. Arun P. Wiita, Raul Perez-Jimenez, Kirstin A. Walther, Frauke Grater, B. J. Berne, Arne Holmgren, Jose M. Sanchez-Ruiz, and Julio M. Fernandez. NATURE, 450, 124-130, (2007) (pdf)Download

Force-Clamp Spectroscopy of Single-Protein Monomers Reveals the Individual Unfolding and Folding Pathways of I27 and Ubiquitin. Sergi Garcia-Manyes, Jasna Brujić, Carmen L. Badilla, and Julio M. Fernandez. BIOPHYS. J. (pdf)Download

Signatures of hydrophobic collapse in extended proteins captured with force spectroscopy. Kirstin A. Walther, Frauke Grater, Lorna Dougan, Carmen L. Badilla, Bruce J. Berne, and Julio M. Fernandez. PNAS, 104, 7916 –7921 (2007) (pdf)Download

Dwell-Time Distribution Analysis of Polyprotein Unfolding Using Force-Clamp Spectroscopy. Jasna Brujic, Rodolfo I. Z. Hermans, Sergi Garcia-Manyes, Kirstin A. Walther, and Julio M. Fernandez. BIOPHYS. J. 92, 2896–2903 (2007) (pdf)Download

The Mechanical Fingerprint of a Parallel Polyprotein Dimer. Atom Sarkar, Sofia Caamano, and Julio M. Fernandez. BIOPHYS. J. 92, L36–L38. (2007) (pdf)Download

Contour Length and Refolding Rate of a Small Protein Controlled by Engineered Disulfide Bonds. Sri Rama Koti Ainavarapu, Jasna Brujic, Hector H. Huang, Arun P. Wiita, Hui Lu,z Lewyn Li, Kirstin A. Walther, Mariano Carrion-Vazquez, Hongbin Li, and Julio M. Fernandez. BIOPHYS. J. 92, 225–233 (2007) (pdf)Download

Mechanical Unfolding Pathways of the Enhanced Yellow Fluorescent Protein Revealed by Single Molecule Force Spectroscopy. Raul Perez-Jimenez, Sergi Garcia-Manyes, Sri Rama Koti Ainavarapu, and Julio M. Fernandez. J. BIOL. CHEM. 281, 52, (2006) (pdf)Download

Force-dependent chemical kinetics of disulfide bond reduction observed with single-molecule techniques. Arun P. Wiita, Sri Rama Koti Ainavarapu, Hector H. Huang, and Julio M. Fernandez. PNAS, 103, 7222–7227 (2006) (pdf)Download

Sub-Angstrom Conformational Changes of a Single Molecule Captured by AFM Variance Analysis. Kirstin A. Walther, Jasna Brujic, Hongbin Li, and Julio M. Fernandez. BIOPHYS. J. 90, 3806–3812 (2006) (pdf)Download

Single-molecule force spectroscopy reveals signatures of glassy dynamics in the energy landscape of ubiquitin. Jasna Brujic, Rodolfo I. Hermans . Kirstin A. Walther, and Julio M. Fernandez. NAT. PHYS. 21, 282-286 (2006) (pdf)Download

Stepwise Unfolding of Ankyrin Repeats in a Single Protein Revealed by Atomic Force Microscopy. Lewyn Li, Svava Wetzel, Andreas Pluckthun, and Julio M. Fernandez. BIOPHYS. J. 90: L30–L32. (2006) (pdf)Download

Ligand Binding Modulates the Mechanical Stability of Dihydrofolate Reductase. Sri Rama Koti Ainavarapu, Lewyn Li, Carmen L. Badilla, and Julio M. Fernandez. BIOPHYS. J. 89, 3337–3344 (2005) (pdf)Download

Mechanical Unfolding Intermediates Observed by Single-molecule Force Spectroscopy in a Fibronectin Type III Module. Lewyn Li, Hector Han-Li Huang, Carmen L. Badilla and Julio M. Fernandez. J. MOL. BIOL. 345, 817–826 (2005) (pdf)Download

The Elasticity of Individual Titin PEVK Exons Measured by Single Molecule Atomic Force Microscopy. Atom Sarkar, Sofia Caamano, and Julio M. Fernandez. J. BIOL. CHEM. 280, 6261–6264, (2005) (pdf)Download

Response to Comment on ‘‘Force-Clamp Spectroscopy Monitors the Folding Trajectory of a Single Protein’’. Julio M. Fernandez, Jasna Brujic, Hongbin Li. 306, 411c (2004) (pdf)Download

Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave. Atom Sarkar, Ragan B. Robertson, and Julio M. Fernandez. PNAS, 101, 12882–12886 (2004) (pdf)Download

The unfolding kinetics of ubiquitin captured with single-molecule force-clamp techniques. Michael Schlierf, Hongbin Li, and Julio M. Fernandez. PNAS, 101, 7299–7304 (2004) (pdf)Download

Force-Clamp Spectroscopy Monitors the Folding Trajectory of a Single Protein. Julio M. Fernandez, and Hongbin Li. SCIENCE, 303, 1674-1678, (2004) (pdf)Download

Mechanical Design of the First Proximal Ig Domain of Human Cardiac Titin Revealed by Single Molecule Force Spectroscopy. Hongbin Li and Julio M. Fernandez. J. MOL. BIOL. 334, 75–86, (2003) (pdf)Download

The Force-Driven Conformations of Heparin Studied with Single Molecule Force Microscopy. Piotr E. Marszalek, Andres F. Oberhauser, Hongbin Li, and Julio M. Fernandez. 85, 2696–2704 (2003) (pdf)Download

The mechanical stability of ubiquitin is linkage dependent. Mariano Carrion-Vazquez, Hongbin Li, Hui Lu, Piotr E. Marszalek, Andres F Oberhauser, and Julio M. Fernandez. NAT. STRUCT. MOL. BIOL. 9, 738-743, (2003) (pdf)Download

Linkage between ATP Consumption and Mechanical Unfolding during the Protein Processing Reactions of an AAA Degradation Machine. Jon A. Kenniston, Tania A. Baker, Julio M. Fernandez, and Robert T. Sauer. CELL, 114, 511–520, (2003) (pdf)Download

Reverse engineering of the giant muscle protein titin. Hongbin Li, Wolfgang A. Linke, Andres F. Oberhauser, Mariano Carrion-Vazquez, Jason G. Kerkvliet, Hui Lu, Piotr E. Marszalek, and Julio M. Fernandez. NATURE, 418, 998-1002, (2002) (pdf)Download

The Mechanical Hierarchies of Fibronectin Observed with Single-molecule AFM. Andres F. Oberhauser, Carmelu Badilla-Fernandez, Mariano Carrion-Vazquez, and Julio M. Fernandez. J. MOL. BIOL. 319, 433–447 (2002) (pdf)Download

Chair-boat transitions in single polysaccharide molecules observed with force-ramp AFM. Piotr E. Marszalek, Hongbin Li, Andres F. Oberhauser, and Julio M. Fernandez. PNAS, 99, 4278–4283 (2002) (pdf)Download

PEVK Domain of Titin: An Entropic Spring with Actin-Binding Properties. Wolfgang A. Linke, Michael Kulke, Hongbin Li, Setsuko Fujita-Becker, Ciprian Neago, Dietmar J. Manstein, Mathias Gautel, and Julio M. Fernandez. J. STUCT. BIOL. 137, 194–205 (2002) (pdf)Download

Multiple conformations of PEVK proteins detected by single-molecule techniques. Hongbin Li, Andres F. Oberhauser, Sambra D. Redick, Mariano Carrion-Vazquez, Harold P. Erickson, and Julio M. Fernandez. PNAS, 98, 10682–10686 (2001) (pdf)Download

Single molecules. Th. Basche, S. Nie, and J. M. Fernandez. PNAS, 98, 10527–10528 (2001) (pdf)Download

Unfolding of Titin Domains Explains the Viscoelastic Behavior of Skeletal Myofibrils. Ave Minajeva, Michael Kulke, Julio M. Fernandez, and Wolfgang A. Linke. BIOPHYS. J. 80, 1442–1451 (2001) (pdf)Download

Fingerprinting polysaccharides with singlemolecule atomic force microscopy. Piotr E. Marszalek, Hongbin Li, and Julio M. Fernandez. NAT. BIOTECH. 19, 258-262, (2001) (pdf)Download

Stepwise unfolding of titin under force-clamp atomic force microscopy. Andres F. Oberhauser, Paul K. Hansma, Mariano Carrion-Vazquez, and Julio M. Fernandez. PNAS, 98, 468–472 (2001) (pdf)Download

Point mutations alter the mechanical stability of immunoglobulin modules. Hongbin Li, Mariano Carrion-Vazquez, Andres F. Oberhauser, Piotr E. Marszalek and Julio M. Fernandez. NAT. STRUCT. BIO. 7, 1117-1120, (2000) (pdf)Download

Single Molecule Force Spectroscopy of Modular Proteins in the Nervous System. Thomas E. Fisher, Mariano Carrion-Vazquez, Andres F. Oberhauser, Hongbin Li, Piotr E. Marszalek, and Julio M. Fernandez. NEURON. 27, 435–446 (2000) (pdf)Download

Stretching single molecules into novel conformations using the atomic force microscope. Thomas E. Fisher, Piotr E. Marszalek and Julio M. Fernandez. NAT. STRUCT. BIOL. 7, 719-724 (2000) (pdf)Download

Intracellular Ca+2 channel immunoreactivity in neuroendocrine axon terminals. Thomas E. Fisher, Mariano Carrion-Vazquez, Julio M. Fernandez. FEBS LETT. 482, 131-138 (2000) (pdf)Download

Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering. Mariano Carrion-Vazquez, Andres F. Oberhauser, Thomas E. Fisher, Piotr E. Marszalek, Hongbin Li, Julio M. Fernandez. PROG. BIOPHYS. MOL. BIOL. 74, 63–91 (2000) (pdf)Download

Atomic force microscopy captures quantized plastic deformation in gold nanowires. Piotr E. Marszalek, William J. Greenleaf, Hongbin Li, Andres F. Oberhauser, and Julio M. Fernandez. PNAS, 97, 6282–6286 (2000) (pdf)Download

Atomic force microscopy reveals the mechanical design of a modular protein, Hongbin Li, Andres F. Oberhauser, Susan B. Fowler, Jane Clarke, and Julio M. Fernandez. PNAS, 97, 6527–6531 (2000) (pdf)Download

Mechanical unfolding intermediates in titin modules. Piotr E. Marszalek, Hui Lu, Hongbin Li, Mariano Carrion-Vazquez, Andres F. Oberhauser, Klaus Schulten, and Julio M. Fernandez. NATURE, 402, 100-103 (1999) (pdf)Download

Single protein misfolding events captured by atomic force microscopy. Andres F. Oberhauser, Piotr E. Marszalek, Mariano Carrion-Vazquez. and Julio M. Fernandez. NAT. STRUCT. BIOL. 6, 1025-1028, (1999) (pdf)Download

The study of protein mechanics with the atomic force microscope. Thomas E. Fisher, Andres F. Oberhauser, Mariano Carrion-Vazquez, Piotr E. Marszalek and Julio M. Fernandez. TIBS, 24, 379-384 (1999) (pdf)Download

Pulsed Laser Imaging of Ca21 Influx in a Neuroendocrine Terminal. Thomas E. Fisher and Julio M. Fernandez. J. NEUROSCI. 19, 7450–7457 (1999) (pdf)Download

Atomic force microscopy captures length phenotypes in single proteins. Mariano Carrion-Vazquez, Piotr E. Marszalek, Adnres F. Oberhauser, and Julio M. Fernandez. PNAS, 96, 11288–11292 (1999) (pdf)Download

Atomic levers control pyranose ring conformations. Piotr E. Marszalek, Yuan-Ping Pang, Hongbin Li, Jamal El Yazal, Andres F. Oberhauser, and Julio M. Fernandez. PNAS, 96, 7894–7898, (1999) (pdf)Download

The micromechanics of single molecules studied with atomic force microscopy. Thomas E. Fisher, Piotr E. Marszalek, Andres F. Oberhauser, Mariano Carrion-Vazquez, and Julio M. Fernandez. J. PHYSIOL. 520-514 (1999) (pdf)Download

Mechanical and chemical unfolding of a single protein: A comparison. Mariano Carrion-Vazquez, Andres F. Oberhauser, Susan B. Fowler, Piotr E. Marszalek, Sheldon E. Broedel, Jane Clarke, and Julio M. Fernandez. PNAS, 96, 3694–3699, (1999) (pdf)Download

Polysaccharide elasticity governed by chair±boat transitions of the glucopyranose ring. Piotr E. Marszalek, Andres F. Oberhauser, Yuan-Ping Pang, and Julio M. Fernandez. NATURE, 396, 661-664 (1998) (pdf)Download

Elastically Coupled Two-Level Systems as a Model for Biopolymer Extensibility. Matthias Rief, Julio M. Fernandez, and Hermann E. Gaub. PHYS. REV. LETT. 81, 4764-4767 (1998) (pdf)Download

The molecular elasticity of the extracellular matrix protein tenascin. Andres F. Oberhauser, Piotr E. Marszalek, Harold P. Erickson, and Julio M. Fernandez. NATURE, 393, 181-185 (1998) (pdf)Download

Regulation of Exocytotic Fusion by Cell Inflation. Carles Solsona, Barbara Innocenti, and Julio M. Fernandez. BIOPHYS. J. 74, 1061–1073 (1998) (pdf)Download

Reversible Unfolding of Individual Titin Immunoglobulin Domains by AFM. Matthias Rief, Mathias Gautel, Filipp Oesterhelt, Julio M. Fernandez, Hermann E. Gaub. SCIENCE, 276, 1109-1112, (1997) (pdf)Download

Cellular and molecular mechanics by atomic force microscopy: Capturing the exocytotic fusion pore in vivo? J. M. Fernandez. PNAS, 94, 9–10, (1997) (pdf)Download

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